We used different protocols and DNA polymerases for different goals and type of PCRs. The different type of PCRs are summarized
A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast - ScienceDirect
How to Calculate the Tm and Ta of the primers after adding attB sites? | ResearchGate
NEBtools - Apps on Google Play
Molecular Cloning Technical Guide
NEB Tm Calculator
Q5 Hot Start High-Fidelity 2X Master Mix (M0494) - New England Biolabs Japan
Q5® High-Fidelity DNA Polymerase | NEB
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IJERPH | Free Full-Text | Development and Piloting of a Web-Based Tool to Teach Relative and Absolute Risk Reductions
Q5® High-Fidelity DNA Polymerase | NEB
The DNA primer melting temperature (Tm) - YouTube
EBWS: Essential Bioinformatics Web Services for Sequence Analyses
Figure 1 from Multiplex PCR using Q5® High-Fidelity DNA Polymerase | Semantic Scholar
NEB Tm Calculator
Mutation Maker, An Open Source Oligo Design Platform for Protein Engineering | ACS Synthetic Biology
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NEB Interactive Tools - New England Biolabs GmbH
The DNA primer melting temperature (Tm) - YouTube
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Phusion DNA Polymerase - New England Biolabs GmbH
